Human F-hb (Free Haemoglobin) Elisa Kit

We are engaged in offering excellent quality Human f-Hb (Free Haemoglobin) ELISA Kit to our valuable clients.
Detection Range : 6.25-400ng/mL
Sensitivity : 3.75 ng/mL

This kit recognizes natural and recombinant Human f-Hb. No significant cross-reactivity or interference between Human f-Hb and analogues was observed. Notei Limited by existing techniques, cross-reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between Human f-Hb and all the analogues.

Test principle:-
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human f-Hb.Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human f-Hb and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human f-Hb, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm A 2 nm. The OD value is proportional to the concentration of Human f-Hb.