Next Generation Antibody Sequencing

Our organization hold expertise in providing services of Next Generation Antibody Sequencing. Antibody experts have developed a novel strategy to determine the complete sequence of an antibody with unparalleled speed and accuracy. The technique is called a Database Assisted Shotgun Sequencinga (DASS) and combines all the advantages of existing methods. Antibody sequencing has now become a routine measurement that typically can be completed within 10 working days for IgGs. However, the method is applicable for all antibody formats, namely IgMs, fluorochrome conjugates, immobilized antibodies and mixtures.

The Technique
In the first step your antibody is fragmented to peptides by a special technique, that generates up to 5000 different peptides per chain. This set of peptides will be analyzed by high end mass spectrometers to generate extensive sequence information. MS/MS spectra are then de Novo sequenced by the latest algorithms and matched against a database with related sequences. Special a in housea data mining tools allow us to extract the sequence information from tenthousends of MS/MS spectra within hours.
Sequencing of the V and J and C segments by "Database Assisted Shotgun Sequencing"

The V and J and C gene segments of antibodies are available in public databases (germline sequences, see figure 1). However, during the maturation of an antibody, the B-cell introduces hypermutations into the sequence to optimize the affinity.