Hcv Igg Elisa Kit

These HCV IgG ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for the qualitative determination of antibody to Hepatitis C Virus in human serum or plasma.
COMPONENTS
No. Components Volume Quantity
1 Microplate 8 x 12 well 1 plate
2 Positive Control 0.5 ml 1 vial
3 Negative Control 0.5 ml 1 vial
4 Conjugate 6 ml 1 vial
5 Sample Diluent 11 ml 1 vial
6 Wash Buffer (25x) 30ml 1 vial
7 Substrate Solution A 6 ml 1 vial
8 Substrate Solution B 6 ml 1 vial
9 Stop Solution 6 ml 1 bottle
10 Plate Covers piece 2 pcs
11 Instruction piece 1 pc

ANALYTICAL PERFORMANCE CHARACTERISTICS
1. Sensitivity
The sensitivity studies have been made on a panel of 738 positive samples. DAAN HCV has presented a 99.8% sensitivity on true HCV positive panels.
2. Specificity
The specificity has been determinated on blood donors samples (2318 samples tested) and on hospitalized patients (348 samples tested). The specificity calculated is 98.9% and 98.5% respectively.
3. Precision
Assay reproducibility was determined by assaying 3 specimens in replicates of 10 on 2 consecutive runs using the same production lot. The Intra-assay and Inter-assay standard deviation (S.D.) and the coefficient of variation (%CV) were calculated (Table I).The mean signal to cut-off ratio (S/CO) is defined as the Mean Sample Absorbance divided by the calculated Cut-Off value.