Protein Expression In Mamalian Cells

We are the leading service provider involved in rendering services of Protein Expression in Mamalian Cells. Creative BioLabsa s proprietary HostOptimTM technology enables generation of high yielding production systems across different industry relevant platforms (e.g. CHO, HEK, Pichia pastoris).

Mammalian cells have the ability to perform most comprehensive post-translational modifications and to secrete glycoproteins that are correctly folded and contain complex antennary oligosaccharides with terminal sialic acid. Mammalian cells are widely used in the production of recombinant proteins, antibodies, virus, viral-subunit proteins, and gene-therapy vectors.

Protein scientists at Creative BioLabs have experience and expertise in both transient and stable expression using a multitude of cell lines, including CHO and HEK293 expression systems. To meet different needs for protein expression, mammalian cell protein expression can be carried out via the following different approaches:
Transient burst expression
Adenovirus-assisted expression
Stable mammalian cell line expression (CHO and HEK293)

CHO expression systems
CHO cells are the most commonly used mammalian cells for protein expression. Our homogeneous stable super-producer clones are obtained without cloning in just one week, whereas classical generation of stable cell lines takes 6 to 9 months. The obtained cell lines can be banked and re-used for further productions.