Immunoglobulin G Test Kit (Immunoturbidimetric Assay), Igg

The Immunoglobulin G Test Kit (Immunoturbidimetric Assay), IGG is a quantitative turbidimetric test for the measurement of IgG in human serum or plasma.
Anti-human IgG antibodies when mixed with samples containing IgG, form insoluble complexes. These complexes cause an absorbance change, dependent upon the IgG concentration of the patient sample, that can be quantified by comparison from a calibrator of know IgG concentration.
For in vitro diagnostic use only.a Applied analyzera
Spectrophotometer or biochemical analyzer with 340nm wavelength.
a Samplea
Fresh serum or plasma. Do not use highly hemolized or lipemic samples. If not determine in time, store at -20 a . Avoid repeat freezing and melting.
a Procedurea
1.Assay methodi two pointi wavelength 340nm
2. Application on chemistry analyzer:
SampleReagenti R1/R2i TemperatureReaction timei T1/T2i
3I L200I L/100I L37a 300s./ 300s.

UnitReaction Direction Standard curve simulation formula
g/LPositiveMulti point calibration, non-linearity calibration mode
3.Determination: Add 3uL samples into 200I L R1,mix thoroughly and incubate the cuvette at 37a for 300 seconds. Read the absorbance A1 against the blank.. Then mix 100I L R2 thoroughly and incubate the cuvette for 300 seconds, read the absorbance A2. The absorbance of complex is a Ai A2-A1i .
Note: different chemistry analyzer has own specifications, but should be subject to the basic specifications herein above, and the kit could be used after setting on the chemis